Federal Civil Procedure

I. Personal Jurisdiction – in what state can the P sue the D? a. Two step-analysis i. Satisfy a statute AND ii. Satisfy the constitution (due process) b. In Personam Jurisdiction – jurisdiction over person, not property, b/c of some contact b/t D and forum state i. Statutory Analysis 1. Every state has statutes allowing jurisdiction based on domicile, presence instate when served w/ process, and consent (implied or actual). 2. Long-arm statute ( allows jurisdiction over non-residents ii. Constitutional Analysis (International Shoe) 1. Test ( Does D have â€Å"such minimum contacts w/ the forum so that exercise of jurisdiction does not offend traditional notions of fair play and substantial justice† 2. Factors a. Contact – some tie b/t D and forum i. Purposeful Availment – D’s voluntary act 1. i. e. D ships goods into forum state or D uses roads or causes effects in forum state ii. Foreseeability – D would get sued in this forum b. Fairness i. Relatedness – b/t contact and the claim 1. not always necessary to have relatedness if have substantial ties w/ the forum a. i. e. D domiciled there, business there, served w/ process there can be sued in that state under general jurisdiction ii. Convenience – forum ok unless puts D at a severe disadvantage in the litigation iii. State’s interest – provide forum for its citizens TIP: My parents frequently forgot to read childrens’ stories M – minimum contacts P – purposeful availment F – foreseeability F – fairness R – relatedness C – convenience S – state’s interest II. Subject Matter Jurisdiction – in what court? Federal courts only hear two types of cases: diversity of citizenship and federal question a. Diversity of Citizenship Cases i. Citizens of different states 1. Complete diversity rule ( no diversity if ANY P is a citizen of the same state as any D, at the time the case is filed 2. Citizenship a. Human – can only have one place of citizenship i. Domicile – 1. presence instate AND 2. subjective intent to make permanent home b. Corporation – can have more than one place of citizenship i. State where incorporated AND ii. One state principal place of business 1. Only one PPB a. Headquarters OR i. Most use this to designate unless all activity is in one state b. Most production or service activity c. Un-incorporated associations i. Use citizenship of ALL ITS MEMBERS d. Decedents, minors, incompetents i. Look to their citizenship NOT the representative’s citizenship ii. Amount in controversy – good faith allegation the claim in the complaint exceeds $75,000. 00, exclusive of interest and costs 1. Aggregation – adding together two or more claims to meet amount in controversy requirement a. Need one P and one D b. Joint tortfeasors – use total value of claim, irrelevant of the # of parties 2. Equitable Relief – if either test met, then it’s OK a. P’s viewpoint: does injunction cover loss of value by more than $75k? OR b. D’s viewpoint: would it cost D more than $75k to comply w/ the injunction b. Federal Question – claim â€Å"arises under† federal law c. Supplemental Jurisdiction – no federal jurisdiction b/c no diversity or FQ, BUT may still be able to get into federal court i. Test 1. common nucleus of operative fact – arise out of same transaction or occurrence as underlying claim ii. Limitation 1. can not use to overcome lack of diversity in a diversity of citizenship case BUT 2. Can use to overcome lack of diversity in a FQ case 3. Can use to overcome amount in controversy in diversity cases d. Removal – allows D’s to have case filed in state court â€Å"removed† to federal court i. What cases? – if case could be heard in federal court ii. Where? – ONLY to the federal district court embracing the state court iii. When? – no later than 30 days after service of the first removable document e. Erie Doctrine III. Venue IV. Service of Process V. Pleadings VI. Joinder of Parties VII. Discovery VIII. Pretrial Adjudication IX. Conferences and Meetings X. Trial, Judgment and Post-trial Motions XI. Appeal XII. Claim and Issue Preclusion

Mary Ainsworth

When reading many introductions on the history of psychology it is noticed there are very few females mentioned. That does not mean women are not attributed to making significant impacts in the development of psychology. â€Å"The contributions of many of psychology's most eminent female thinkers have long been ignored, but that is starting to change as more history texts begin to recognize women such as Karen Horney, Mary Ainsworth, Leta Hollingworth, and Christine Ladd-Franklin in their pages. † These women are just a few who have made tremendous contributions and marks on psychology.The background, theoretical approach, and contributions of Mary Ainsworth are very significant to the field psychology even still today. Time line/Background of Mary Ainsworth Mary Ainsworth was born in December 1913 in Glendale, Ohio. She was the oldest of three girls; in 1918 her family relocated to Toronto Canada, and gained their citizenship. In her household education and studies were noted to be important. â€Å"When she was fifteen, she read William McDougall's book Character and the Conduct of Life, which inspired her lifelong interest in psychology† (O'Connell, 1983).In 1929 Ainsworth attended study at the University of Toronto; she was one of only four students to finish the honors degree program in psychology. At the time her father decided it would be best for her to become a stenographer, but he was still supportive of her pursuit of graduate work in psychology. In 1939Mary Ainsworth was a recent Ph. D. graduate. She wanted to stay at the University of Toronto, and she fascinated the head of the psychology department. However, she was not selected for a position because the University Senate refused to appoint a female.In 1942 Ainsworth joined the Canadian Women’s Army Corp, and after serving as a counselor in the Army for four years, she came back to the University of Toronto and gained the position assistant professor. She got engaged to Leonar d Ainsworth a graduate student, and they married in 1950. It was difficult working as assistant professor on the faculty where her husband was a pupil so both moved to London, England. â€Å"Mary Ainsworth was selected for a research position at the Tavistock Clinic under psychiatrist John Bowlby.Bowlby’s research of the effects of separation of children from their mother’s/caregiver’s served as a precursor of Ainsworth’s earlier work on the security theory†. In 1953 Leonard Ainsworth was interested in going to Africa. Mary Ainsworth could find employment as a research psychologist at the East African Institute of Social Research in Kampala, Uganda. She conducted a short-term naturalistic study of the mother-infant relationship and published the results.Two years later Mary landed a position as a lecturer in Baltimore, Maryland, at John Hopkins. Not only did she lecture, and supervise students, she set up a private practice dedicated to children. I n 1960 because of divorce Ainsworth became very depressed. In 1963, one year after starting the research she is best known for she became a full professor. In1975 Ainsworth left Hopkins for a professor position at the University of Virginia. She taught there until her retirement in 1984. She remained active in her profession until 1992.The American Psychological Foundation awarded her the Gold Medal Award for life achievement in the science of psychology from. In 1999 Mary Ainsworth passed at the age of 86, she never had any children but her major contributions were in study of children. Theoretical Perspective of Mary Ainsworth Bowlby and Ainsworth worked together to develop the attachment theory and research. â€Å"The distinguishing characteristic of the theory of attachment that we have jointly developed is that it is an ethological approach to personality development.Although they had separate approaches to understanding personality development, they worked together each addin g different ideas and perspectives. In Uganda Ainsworth spent time doing research on mother child interactions. At the same time she teaching and lecturing about psychology at John Hopkins, Mary Ainsworth began work to create a test to measure attachments between mother’s and caregiver’s, and their children. Here she developed the â€Å"Strange Situations† assessment. Children ages 12 months-18 months were observed during the assessment.A researcher watched a child’s reaction when he or she was briefly left alone in an unfamiliar room. Important information was revealed during the separation and upon the mother’s/caregiver’s return. â€Å"Based on her observations, Ainsworth concluded that there are three main attachment styles. The three main attachment styles are secure, anxious- avoidant, and anxious resistant†. Because her initial finding, her work has spawned numerous studies into the nature of attachment and the different attach ment styles that exist between children and caregivers.Mary Ainsworth’s contributions to psychology Significant contributions to the science of psychology have been made by Mary Ainsworth with her â€Å"Strange Situations† assessment. After the research she concluded the main attachment styles are secure, anxious-avoidant, and anxious-resistant. She set a platform and many others shortly followed. Her controversial research on attachment played an important role in understanding the development of children.In 1986 researchers Main and Solomon added a fourth attachment style: disorganized-insecure. There are numerous studies that support Ainsworth’s research. Additional research has also shown early attachment styles can help predict behaviors later in life. Mary Ainsworth’s research and contributions are still important to the study of psychology today. Conclusion â€Å"Mary Ainsworth knew her work was debatable and could be understood by some in the wom en’s movement as a order to mothers to stay home with their children in their early age†.â€Å"And while I emphasize the importance of a secure attachment between infant and caregiver, and that full-time mothering may be the usual way of ensuring a secure attachment, she did not deny that alternative arrangements were possible. She said, â€Å"Had I myself had the children for whom I vainly longed, I like to believe I could have arrived at some satisfactory combination of mothering and a career, but I do not believe that there is any universal, easy, ready-made solution to the problem† (Ainsworth, 1983. p. 216).With tables turning and the contributions of psychology’s most prominent female theorist being added to text books students will study more about the contributions of Mary Ainsworth. Her background, significant contributions, and theoretical approach are vital still today. Many psychology researchers use the â€Å"Strange Situation† assessme nt as a basis for analysis on child development research. Mary Ainsworth lived 86 years and most of her life was spent researching, lecturing, teaching, and observing in the psychology field.

Healthcare in the United States a Social Problem Essay

Healthcare in the United States a Social Problem - Essay Example The total nationalized health expenditures have arisen to 6.9 % in the fiscal year of 2007-2008 that is two times the inflation rate of U.S. economy. In 2007-08, the totality expenditure on the health care was estimated at a hefty sum of $2.3 trillion i.e. $7600/individual. The Total health care expenditure comprises of approx. 17 % of the gross domestic product (GDP) of United States of America. As per the various studies conducted by the experts, it has been anticipated that by the year 2016, the U.S. health care expenditure will boost up at the related levels for the subsequent decade attaining at least the $4.2 trillion which would be the over 21% of the United States future GDP. During the fiscal year 2007-08, the employer health insurance premiums augmented up to 6.1 % which is double of the inflation rate. The yearly premium cost for an employer health plan usually covering a family unit of four person’s averages near $12,100. The annual premium for the sole coverage is approximately more than $4,600. According to the experts the health care system of U.S. is puzzled with the in-efficient system, too much administrative operating expense, exaggerated costs, deprived management and unsuitable concern, misuse and deceptions. All these problems drastically amplify the cost of therapeutic care and health insurance for the company & staff and also influence the protection chances of their families. Health care in the United States is in catastrophe condition. The prosperous country on globe pays out 1/7 of its capital on health care and still leaves approximate 18 % of its population uninsured. The old aged people are often required to opt between foodstuff and pharmaceuticals. Several have deserted bio-medicine overall for the Complementary and substitute medications utilized by a lot of Americans in the previous centuries. According to the IOM {Institute of Medicine of the National Academy of Sciences}, the United States is the lone

Carl Friedrich Gauss Essay Example | Topics and Well Written Essays - 1000 words

Carl Friedrich Gauss - Essay Example Gauss represented a clear expression of a great mathematician of a small town called Gottingen. He is known in history for his remarkable geometrical discoveries. He is known for his discoveries in method of least squares, quadratic reciprocity, and non-Euclidean geometry. One of his greater works is also seen in astronomy. I totally agree with the works of Gauss on construction of polygons, least squares method, the fundamental theorem of algebra or the non-Euclidean's - differential geometry. Though he never published these discoveries anywhere but his work is highly remarkable. Gauss started with these discoveries at a very early age. He proved the construction of regular 17 sided polygons called heptadecagon. He proved that this can be constructed simply with the help of a ruler and a compass and thinks this is one of his greatest achievements in the history of geometry. Because as opposed to Kepler, Gauss proved that not only a triangle, square, pentagon, hexagon are constructible but then he proved it right that 17 sided figures can also be constructed with the equal lengths. He further added that 17 gon can be constructed using four quadratic equations (Swetz, 1994). One more important discovery of Gauss is the theory of least squares and normal distribution. He proved that every curve led to the least squares. He believed that the problems can be simplified by solving the errors evenly distributed. As a result, this gave the accurate estimates by solving the errors incurred in the equation. The construction was possible with trigonometric functions along with arithmetic and square roots. Gaussian distribution curve is a bell shaped curve used for normal distribution. In the Gaussian distribution, all the values combined give the value as 1. Gauss gave the fundamental theorem of algebra where he proved that any algebraic equation to the degree n, where n is a positive integer will have n number of roots. I totally agree with Gauss in his work on Disquisitiones Arithmeticae where he investigated the number theory within mathematics. Also, he made it possible to draw a circle into equal arch's just with the help of a ruler and a compass. In the number theory, he came up with an idea of congruence in numbers with the help of which infinite series of whole numbers can be broken into smaller chunks of numbers. This can e explained by taking an example: 700 - 400 = 300 right. Here the remainder is 300. This remainder can further be divided into smaller chunks of numbers like 100, 50, and 30 and so on. Here 700 and 400 are congruent to each other by modulo 100. This concept was very much popular among the digital watches. The gauss theory of numbers has its relevance even today and many great mathematicians of today hold this o pinion. It plays a crucial role in the Internet world today through security technologies (Struik 1987). In is theory of geometry, he never agreed to Euclidean's indeed known for his non-Euclidean geometry. He found that parallel postulate fails in the Euclid's geometrical theory that through a point which is not on the line, in this case either there is none or more than one parallel line. The basic difference between the Euclid and Non Euclid's theory on geometry was the nature of parallel lines. Non Euclid theory discovered the geometry of space. The non Euclidean's geometry studied Elliptic geometry

Beach Rental Case study Assignment Example | Topics and Well Written Essays - 1250 words

Beach Rental Case study - Assignment Example That will ensure that they have good accommodation and a good environment in which the students can be working from. The efficiency of the company can be done by measuring of the efficiency of workers in the company. The measurement of performance by employees is a critical thing for ensuring that there is success in the running of a business organization. The measurement of performance is done so as to ensure that the employees of a company provide value for the money that they earn. Performance may be measured by the number of hours worked or by the quality of work done or the output that accrues from an individual or group of employees. The report will give details of a number of performance measurement metrics that may be used by the Company. Performance measurement metrics The measurement of the performance of employees is a thing that is advantageous to the company. Performance measurement usually ensures that there is equitable distribution of incentives. The performance measu rement also ensures that there is increase in efficiency and productivity in the company. A performance metric that is effective should ensure that there is a gauge that is accurate and one that would ensure that the employees are valued according to their performance and output in the company. Productivity metrics One of the components of measuring the performance of a company or business is by the use of productivity. Productivity metrics usually measures the amount of work done by an employee within a specified time period. Employees usually have a different learning curve. That means that new employees would be less productive as compared to old and experienced employees. In a manufacturing company, productivity can be measured by the total output of an individual in a specified time period. In a sales department, productivity is usually measured by the total sales made by a single employee. Efficiency metrics Efficiency can be described as an attempt to increase on productivity by utilizing minimum resource or by the use of the most cost-effective method. Efficiency usually deals on cutting on time of production and the costs that are incurred during production. That can be done by the company introducing processes that are efficiency and using quality raw materials that are cheap so as to ensure that there is cost cutting. Training metrics Training among employees usually increase on the performance or productivity of employees. The training of employees would ensure that the employees use the most cost effective working processes. The training efforts should be quantified so as to measure the level of productivity that accrues from the training of employees. Goal setting The use of performance review systems that are collaborative usually ensure that supervisors and their juniors are able to set their own personal goals that should be achieved so as to increase the efficiency of the company or business. That can be termed as management by the use of obj ectives and that can be done so as to individually measure the performance of an employee. The employees of the company should undergo training so as to ensure that they are efficient enough in the execution of their roles in the business. Design process Analysis The first process that is involved in designing the training is analysis. The objectives and goals have to be analyzed so as to be able to develop the

Courtly Love Research Paper Example | Topics and Well Written Essays - 1250 words

Courtly Love - Research Paper Example Christianity advocated for real love between two individuals of opposite sex who eventually ended up marrying and establishing a family together, while prohibiting any form of love, passion or desire by the married couples outside of their marriage. On the other hand, the concept of Courtly Love introduced a completely different perception of love, requiring that married ladies could still develop passion and have romance outside of their marriage, as long as they did not violate the rules of chastity and infidelity (Donaldson, 16). The fundamental principle of Courtly Love was that marriages were arranged, and had nothing to do with love, as long as such marriages brought wealth and power, as well as other material benefits to the participants and their families. Nevertheless, while the Courtly Love was a noble idea meant to allow married people, especially ladies to experience love and romance that they did not get out of their marriages which were not based on love, it eventually resulted into illicit and tragic love affairs that did not follow the laid down rules, as exemplified by William Shakespeare’s â€Å"Romeo and Juliet†. ... Most of such ladies were engaged in marriages for the convenience of their families in protecting their material wealth and power, as opposed to marrying out of love, thus living in marriages that had no happiness and where intimacy and romance did not fully exist, because the marriage partners did not have strong feelings for each other (Capellanus, 44). It is through the rise of the concept of Courtly Love, that such ladies were allowed to develop passion for the knights and courtiers who served within the kingly courts, given a leeway by the rule that marriage did not stop them from loving again and engaging in passionate romance with the knights and the courtiers, as long as they adhered to the rules of maintaining chastity and avoiding infidelity (Boase, 31). Nevertheless, with the leeway to engage in passionate romance and to love the knights and the courtiers, it was inevitable for such romances to develop into real feelings of love and lust for sexual engagements, which event ually saw such ladies engage in illicit love affairs with the knights and courtiers, thus breaking the rules of fidelity and chastity, and eventually ending up in tragedies (Jackson, 243). William Shakespeare’s â€Å"Romeo and Juliet† is one of the love literatures that were inspired b the concept of Courtly Love, which depicts the love triangle involving the ruling house and other revered families fighting for the love of 13-year old Juliet (Shakespeare, 23). The literature is motivated by the arranged marriage concept of the middle ages, where Count Paris, who is a member of the ruling family, seeks to marry Juliet out of an agreement reached

Characteristics that a student displays that make him deserving of a Essay

Characteristics that a student displays that make him deserving of a scholarship from the University - Essay Example Hardworking may be a cliche but it still stands true to this day that a student should indeed be diligent, persevering to attain his objectives in life despite the many hindrances there may be. Despite this, the deserving student is also expected to display a positive attitude that will not only be a positive influence to her self but also to those around him. Such individual should be a team player, able to communicate himself clearly to avoid misunderstandings and able to tackle difficult situations in a group. He should be able to manifest organizational skills, able to put his schedules in check and making his time and energy productive. In addition, one should be loyal as this will affect greatly how he acts in places and circumstances that there are no people looking at him. He should then be trust worthy. Respect is another characteristic a good student should possess because this is one of the inner beauties needed by many people and makes the holder of such value loved and r espected in return. This and all of the aforementioned attitudes are all equally important for a student to own and in addition must display good grooming as outer appearance follows inner values.

SMART Goal Setting Essay Example | Topics and Well Written Essays - 250 words

SMART Goal Setting - Essay Example Through the application of SMART goals, it is easier to set goals that drive change and engage all the stakeholders in initiating change. The SMART goals also help in an assessment of the successes of the organization, and what aspects needs to be changed to attain the EHR objectives (Amatayakul, 2012). Through SMART goal setting in EHR as well, it is evident that an implementation project is possible. After setting the goals, the implementation process becomes easier since a follow up program is easily drawn. The set goals are also practical in a way that the expectations are already set and all support mechanisms are enacted so as to achieve the goals (Amatayakul, 2012). Simply put, the SMART goals setting in EHR has been credited with the ability to increase efficiency in the clinical setting as well as patient care (Amatayakul, 2012). However, challenges such as lack of proper follow up hinder a successful realization of the application of SMART goal setting in the clinical

Active teaching strategy Essay Example | Topics and Well Written Essays - 1000 words

Active teaching strategy - Essay Example The reason for choosing a game is that psychologically we tend to perform better in a competitive environment and also studies have soon shown enhanced brain function with lesser amount of stress when the activity being performed by the person is mentally stimulating rather than tiring Keeping this philosophy into consideration the idea that I like to propose for active learning is that of "ten questions".would have a group of four people, each team will have ten questions to find the answer, the questions will be asked by the team leader of each team by mutual discussion with its other members. The answer to each question will be either yes or no. no phrases or other words will be used to answer the question. Similar questions will not be answered and will not be counted in your question count. Once all ten questions are answered the team must guess the answer if the answer is guessed correctly the team receives 10 points and if the team answers incorrectly no points are award. If t he team guesses the answer before the 10 question limit that team will receive bonus 10 points and 20 in all and if it answers incorrectly it will get -10 points. The team to guess most of the answers correctly wins.extra needed and no set up time is required also due to the ease and playability of this activity this activity can also be exercised by the students among themselves. The other good thing about this sort of exercise is that it applicability is universal and can be used by teachers of every subject. This sort of activity also motivates team work among the students which can prove to be very vital for their profession. PROCEDURE: The whole class will be divided into groups of 3 to 4 members. Each team will them have to guess the procedure by asking question to the teacher. The team will then guess the answer and the team with the most correct answer will be the winner. This team will then be awarded the title of washing wizard. To further stimuli the learning process the members of the winning team will be awarded marks which will be counted in their assignment and then these marks will added in the final tally as well. CONCEPT OF THE ACTIVITY: "People learn in response to need. When people cannot see the need for what is being taught, they ignore it, reject it, or fail to assimilate it any meaningful way. Conversely, when they have a need, then, if the resources are available, people learn effectively and quickly." (Brown and Dugout, 2000, p.136). The concept of the activity is very simple and that is to train the nursing students about the importance of hand washing and due to the nature of this activity this importance can be transferred to the students in a fun manner in which they can learn and remember it as well. ACTIVITY METHOD USED: This activity method that is being used is known as "Kolb's Model of Experiential Learning (Kolb, 1984)". This model of learning follows these steps: 1. Action- The learner performs some type of activity related to the lesson or subject. 2. Reflection- The learner reflects about what they did and what happened as a result of their activity. This can be in one of several forms: free writing, journaling, or small or large group discussions. 3. Knowledge/theory- The learner uses the results of the reflection to develop knowledge and theories, which helps further the learning process because the learner is conceptualizing their own theories, not accepting the theory of the instructor.

Determinants Of Health Essay Example for Free

Determinants Of Health Essay Social determinants of health are the circumstances in which people are born, grow up, live, work, and age, as well as the systems put in place to deal with illness. These circumstances are in turn shaped by a wider set of forces: economics, social policies, and politics.Social determinants of health are factors in the social environment that contribute to or detract from the health of individuals and communities. These factors include, but are not limited to the following: Socioeconomic status,Transportation,Housing,Access to services,Discrimination by social grouping (e. g., race, gender, or class),Social or environmental stressors.Certain factors that contribute to the development of illness are: Poverty,Unemployment,High School Education, Health Insurance. Communicable diseases spread from one person to another or from an animal to a person. The spread often happens via airborne viruses or bacteria, but also through blood or other bodily fluid. The terms infectious and contagious are also used to describe a communicable disease. A communicable disease chain is the chain of infection,which includes: INFECTIOUS DISEASE:Any microorganism that can cause a disease such as a bacterium, virus, parasite, or fungus. RESERVOIR: The place where the microorganism resides, thrives, and reproduces, i.e., food, water, toilet seat, elevator buttons, human feces, respiratory secretions. PORTAL OF EXIT : The place where the organism leaves the reservoir, such as the respiratory tract (nose, mouth), intestinal tract (rectum), urinary tract, or blood and other body fluids. MODE OF TRANSMISSION :The means by which an organism transfers from one carrier to another by either direct transmission (direct contact between infectious host and susceptible host) or indirect transmission (which involves an intermediate carrier like an environmental surface or piece of medical equipment). PORTAL OF ENTRY : The opening where an infectious disease enters the host’s body such as mucus membranes, open wounds, or tubes inserted in body cavities like urinary catheters or feeding tubes. SUSCEPTIBLE HOST :The person who is at risk for developing an infection from the disease.

Judicial Inquiry Into Stephen Lawrence

Judicial Inquiry Into Stephen Lawrence It is argued by Shiner (2010) that the judicial inquiry into the events surrounding the death of Stephen Lawrence amounted to a public declaration of police culpability in not only the surrounding the specific case of Stephen Lawrence but a wider concern with the maltreatment of black and ethnic minority communities more generally. (Shiner 2010; 935). The Macpherson Report identified that the combination of three core deficiencies within the police which contributed to an environment fostering racism: (1) professional incompetence, (2) institutional racism and (3) a failure by senior police officers in exercising their public duty. (Macpherson 1999: 46.1). The Report considered that their findings with regard to institutional racism were symptomatic with not only the Metropolitan Police Service (MPS) but with other Police Services and institutions countrywide. (Macpherson 1999; 6.39). It is agreed by a number of academics that the way in which the police handled the Lawrence investig ation were not particularly racist against the Lawrence family but rather it was symbolic of the way in which the police were treating working class families with a sheer lack of understanding on the part of the police of the experiences and expectations of the black community. (Chan 1996; 115-119 and Phillips and Bowling 2007). The important aspect to emerge from the Report was the impact it would have on the police as an institution in that although Stephen Lawrence was not the first or last unresolved racist murder, it was noted by Reiner (2000) that it crystallised the disastrous ebbing away of black confidence in the police. (Reiner 2000; 79). It represented a turning point for policing in that it prompted the most significant review of the relationship between policing and ethnic communities, questioning how policing could be reflective of the community it serves. (Bowling and Phillips 2007; 546). The Macpherson reported noted some 70 recommendations aimed at improving this relationship between the police and ethnic communities which were implemented in the aftermath of the delivery of the Report. (Home Affairs Committee 2007). A key reforming recommendation was Recommendation 61 which required police to keep records of all stop and search activities carried out by the police which would contain the in formation of ethnicity which in turn could be used to determine whether the police were stopping any particular ethnicity disproportionately. (Macpherson 1999; 47.61). The effect of requiring police officers to record the informal aspects of their use of stop and search was one way in which the police could be held to account publically if they disproportionately stopped any ethnicity over another. Additionally, it had sought to reduce the arbitrariness of the use of stop and search by ensuring the police would only use the power appropriately when they had to record each time who they stopped. (Saunders and Young 2010; 97 101). One of the core concerns of the Macpherson Report identified that the police in practice were in effect over-policing particular ethnic communities and by requiring the police to report on their use of discretionary police powers would create an accountability mechanism within which the public could scrutinise the use of police powers. (Macpherson 1999; 6.3 4). It held the potential of becoming the yardstick indicator of policing. However, it is important to note that whilst the Macpherson Report clearly set about to place policing within a new agenda of accountability and openness to the community in which they served, it has been argued by Savage that the police as an institution has been able to undermine, frustrate and withstand any external proposals for reform. (Savage 2003; 171). In addition, it is important to understand the internal police perception of the Macpherson Report in that their reaction was deeply divided between senior ranking officer and rank and file field officers. (Rowe 2004). The senior officers were more open to change and accepted the findings of the Macpherson Report, however, the rank and file field officers protested that the Report was an affront to their professionalism in exercising their public duty. (McLaughlin 2007). Media reporting in 2009 from across a section of sources attempt to argue that the problems emerging from the Macpherson Report were now no longer relevant ten years after Macpherson due to the reforms implemented from the Report. In January 2009 the chair of the Equality and Human Rights Commission, Trevor Phillips wrote in the Daily Mail that the police were no longer institutionally racist. (19 January 2009). Additionally, Jack Straw who was the Secretary of State for Justice also backed the claim that the police had moved on by addressing the problems established in the Macpherson Report. (The Guardian, 23 February 2009). At the tenth anniversary of the Macpherson Report, the Commissioner of the Metropolitan Police Service, Sir Paul Stephenson, also told the media that the police had made significant progress in addressing the concerns of the Macpherson Report. (The Guardian, 24 February 2009). There a number of ways or indicators to assess how successful the Macpherson Report was at delivering a more open and accountable police force since its delivery over ten years ago. The London School of Economics (LSE) through its Mannheim Centre for Criminology commissioned a large number of national surveys of the police between 2002 and 2004 which could act as a good indicator of whether reform was successful. In their report the authors conclude that a number of successes can be claimed since the delivery of the Macpherson Report. (Foster et al 2005). In specific the LSE noted that the Macpherson Reported represented an important mechanism for change which was evidenced through improvements in police responses to hate crimes and in the recording and monitoring of racist incidents. (Ibid; vii ix). Policing structures now allowed for dedicated teams of officers for racial crimes who undertook specialist training in developing specific skills for investigating and managing racial c rimes. However, the LSE Report did note that many officers view these teams with scepticism and often marginalised the officers within them. (Ibid; 92). This highlights that whilst efforts were made at reforming the institutional structure towards creating specialist teams for specific crimes affecting ethnic minorities many existing officers did not support this development. (Buchanan 2006; 173-174). Additionally, the LSE Report noted considerable improvements in murder investigations with considerable improvements on communication between the police and local ethnic communities. It was the creating of community liaison officers which represented a positive change towards creating and fostering an environment of respect between the police and local ethnic communities. These officers allowed the facilitation of police policy to be communicated to local ethnic minorities and also for the police to take account of any concerns within the local ethnicity minority community. Interesting ly, all police forces across the UK have engaged training officers in Community Race Relations to develop existing and new officers skills set in being aware of the diverse cultures and experiences of minority communities within the UK. An important issue raised by the LSE Report was that some police officers within the survey believed that the Macpherson Report was wrong in concluding on the racism within policing and tended to view the problems raised by the Report as being primarily around incompetence. (Foster et al 2005; 33). The danger here is that there is a risk that the real message surrounding the racism issue within policing can become lost within the institutional structure when some officers only focus on the actual incompetence findings within Macpherson. The LSE Report also found that the police use of language and their attempts to abolish the use of racist language amongst officers were becoming successful. (Ibid; 43). This approach was achieved through continuous education of new recruits and retraining schemes for existing officers, the key to the success of this hinged upon the senior officers creating an environment within policing which did not accept the use of racist language. Although the Macpherson Report opened the possibility of effecting real change within policing there still exists a number of key concerns. The concerns revolve around two core issues, firstly police use of stop and search powers and secondly the unsuccessful recruitment of sufficient officers from ethnic communities in order to make the police more reflective of the community it serves. The reporting structure recommended by Macpherson shows that a high proportion of people from the Black community are disproportionately stopped by the police under the stop and search powers. This represents that the database of all stop and searches contain 20% more black people than Asian or White people. (Bennetto 2009; 22). Whilst the Black community is over represented in the criminal justice system for criminal offences it does not justify a disproportionate use of police stop and search power. Additionally, a core failing has been the problem with recruiting officers from within the ethnic co mmunities. In an investigative journalism piece by the BBCs Mark Daly (2003) highlights that a significant problem is that there is a serious underrepresentation within senior ranking positions of ethnic minorities which impact upon policy and public perception.

King James 1 and the Church Essay example -- Essays Papers

King James 1 and the Church King James IV, of Scotland, seized the English throne in 1603 after the death of Elizabeth I and became James I of England. He was the son of Mary Queen of Scots and had been King of Scotland since 1567. During his reign, James increased the power of the monarchy making his rule absolute. James I was involved with every area of government. Under his rule Scotland and England were united, the King James Version of the Bible was published, William Shakespeare and various other writers prospered, education thrived, and the American colonies were founded.[1] However, James faced many problems with unifying the government. One of the main problems was the religious conflict existing within the Church of England. Anglicans and Puritans wanted the church organized in separate ways, but King James felt a unified state church would create a more powerful government God had given James the right to rule and therefore non-conformity to religious policies was a sin against God. Although he wanted one state church, James believed compromise and toleration would naturally drive citizens to become members, conforming to the policies of the Church of England. Throughout his reign James attempted to decrease religious tensions, as people hoped to increase their influence and role they played in the decisions of the church. There was a distinct split within the Church of England between the Puritans and the Anglicans. The Puritans were members of the Church of England, wanting reformation. They wished to eliminate of some ceremonies and dogmas closely resembling those of the Catholic Church. Puritans were more devout Christians then regular members. They believed the Sabbath should be strictly observe... ... wasn’t completely unified, James did make changes that improved conditions. Since he wasn’t completely intolerable of other viewpoints he created a strong central government for one of the first times in English histories. Notes 1. Stephen A. Coston, â€Å" King James I of England Interesting Facts† (10/20/00) A.V. Publications Corp. 2. S. J, Houstan, James I, 1st ed.. (Great Britain:Lowe and Brydone, 1973), 16. 3. Houstan, 57. 4. Antonia Fraser, King James I of England, 1st ed. (New York:Alfred A. Knopf, 1975), 104. 5. Fraser, 105. 6. G.B. Harrison, A Jacobean Journal, 1st ed. (London: George Routledge and Sons, 1941), 105 7. Harrison, 106. 8. Fraser, 117. 9. Harrison, 152. 10. Houstan, 61. 11. Fraser, 182. 12. Houstan, 63. 13. Fraser, 182.

Comparing Nature in Wordsworth’s Ruined Cottage, and Coleridge’s Rime o

Comparing the Representation of Nature in Wordsworth’s Ruined Cottage, and Coleridge’s Rime of the Ancient Mariner For most poets of the Romantic Age, nature played an invaluable role in their works. Man’s existence could be affected and explained by the presence and portrayal of the external nature surrounding it. William Wordsworth and Samuel Taylor Coleridge are no different from the other Romantic poets, and their works abound with references to nature and its correlation to humanity. Specifically, Wordsworth’s â€Å"The Ruined Cottage† and Coleridge’s â€Å"The Rime of the Ancient Mariner† share the theme of nature affecting man, although essential differences exist in their ideas regarding how it affects man. These two works are also similar in that they use a storyteller frame to both deliver and reinforce these ideas. In order for the reader to fully appreciate the representation of nature in these two particular poems, it is necessary to supply a little background on each poet. Wordsworth reigns supreme in the nature tradition. His poetry makes tribute to nature in conjunction with examining the human state, while maintaining that the relationship between the two is unbreakable. In his book English Poetry of the Romantic Period, critic J.R. Watson claims â€Å"the finest of Wordsworth’s nature poetry explores the relationship between [man and the world seen in the spirit of love], in the attempt to demonstrate the power of nature in the rescuing of the individual mind from degradation, materialism, selfishness, and despair† (114). Crediting nature with the answer to life, Wordsworth’s philosophy reveals that there can be no greater truth than that found in the simplicity of nature. He pulls from ... ...ompany, Inc., 2000. 422-38. Coleridge, Samuel Taylor. â€Å"Biographia Literaria.† The Norton Anthology of English Literature: The Romantic Period. 7th ed. Ed. M.H. Abrams. New York: W. W. Norton & Company, Inc., 2000. 468-486. Harding, D. W. â€Å"The Theme of ‘The Ancient Mariner.’† Coleridge: A Collection of Critical Essays. Ed. Kathleen Coburn. Englewood Cliffs: Prentice-Hall, Inc., 1967. 51-64. Watson, J.R. English Poetry of the Romantic Period. New York: Longman, Inc. 1985. Wordsworth, William. â€Å"The Ruined Cottage.† The Norton Anthology of English Literature: The Romantic Period. 7th ed. Ed. M.H. Abrams. New York: W. W. Norton & Company, Inc., 2000. 259-70. Wordsworth, William. â€Å"Preface to Lyrical Ballads.† The Norton Anthology of English Literature: The Romantic Period. 7th ed. Ed. M.H. Abrams. New York: W. W. Norton & Company, Inc., 2000. 238-251.

Food reflection Essay

1. Have you ever kept a food journal before? If so, why? I have done a food once when I was in high school. It was my sophomore year in health class. My teacher wanted us to learn about what kind of food we eat. So she made us do it for one weekend. She wanted us to share what we ate and how everyone ate differently. Some people ate healthier and some did not. She made us look at how much we ate and what she should do to eat healthier. 2. Did this food record make you more aware of what you were eating? Did it make you change your eating habits? Why or why not? This food record does make me more aware of what I am eating. Like for that certain day I ate more than the weekdays. On weekdays I would not have time to eat as much as I did because the weekdays I am busy with schoolwork. That day I was with friends during lunch but during dinner I was alone. I don’t think it will make me change my habit on eating. For me, I like to eat different things each day. I do not like to eat the same thing everyday. I like to change things up so I wont get tired of eating the same thing. This is why I feel like I do not need to change my habit because I do change everyday. This also showed me to eat other things. Like it showed that my vegetable and whole grains are low. Which means that I need to eat more things that have them in it than other food. 3. Did your food/drink intake indicate anything about what was going on in your daily life? No I do not feel like it indicates anything about what is going on in my daily life. Like going to the gym to run or walking around the park with friends. Some days when I am lazy, I would sit around and eat food all day long while watching TV. Then other days I would be out with people working and doing activities. I think the only thing the food report shows is the amount of food that is intake but does not show the other activities I do. The food report does not show if I were working at a job. Whether I would be sitting or standing all day. 4. What surprised you about your intake, if anything? One thing that surprised me in the food I ate was the amount of calories that are in some food. I never realized how much could be in one cup or a couple ounces. Usually I do look at the box where it has the calories and the food in it. While looking back at it, it seems as if the calories was not that important, the important thing is to look at the type of food that is in it. Like if there is any grains or meat or vegetable. I looked at the food report and it showed that my vegetables intakes are low. I thought I was fine with the vegetable but I guess I am not. 5. Was your eating pattern for this day usual? Why or why not? This was normal for my weekends. Like I know I can relax and eat as much as I want over the weekend than on the weekdays. On weekdays I am in class and out of class from 8AM to 2PM. If I were not in class I would be at work. So I do not have as much time to eat whatever I want or as much as I want. On a usually weekday, I would get a snack for the morning and then eat at 2 after all my classes are finished. On weekends I would typically eat more. Having a small meal in the morning and two big meals for lunch and dinner. 6. What changes will you make, if any, to your eating patterns after this assignment? Some changes as I said in some of the other questions are to eat more whole grains, eat more vegetables, and take in less refined grains and meat. I need to cut back on some meat because I know if I eat too much I will start to gain weight. While cutting back some of the food, I also need to start to go to the gym more often.

Existentialist traits in works of Henrik Ibsen Essay

Existentialism is a major twentieth century continental European philosophical movement. The label was inspired by the tendency of some of the writers like Kierkegaard, Heidegger , Sartre and Nietzsche to use the term â€Å"existence† for a kind of being or life unique, in their view, to human beings. Only in the case of human beings is the conduct of their life an â€Å"issue† for them; only they can â€Å"stand out† – exstare, the Latin word from which â€Å"exist† comes – from their lives and reflect upon them; and only they have the capacity freely to shape their lives. Though Existentialism can appear in a number of different forms, the focal point as Jean Paul Sartre puts it is â€Å"existence precedes essence†. The existentialists are of the view that as there is not pre-determined course of human life, they are only what they make themselves. This gives human beings a freedom to choose. Human beings have to make choices or are rather â€Å"condemned † to make choices and bear their consequences. Every act is conscious and so all the responsibility falls on their shoulders with no one to rely on and thus give rise to feelings of despair and anxiety. All of which had been sensitively explored earlier in the century by such literary figures as Luigi Pirandello (1867-1936) and Franz Kafka (1883-1924) and can seen to be already an abiding concern in the works of Henrik Ibsen (1828-1906). The Norwegian playwright, Henrik Ibsen a was an advocator of individualism and was against the social norms which shape the lives of human beings. His characters live themselves out in the spirit of reckless and vehement self-assertion – superman and superwomen. Ibsen was a believer in freedom to will. Many of his characters can be seen in this light who reject to be shaped by some force outside their own will. In his famous and controversial play â€Å"A Doll’s House† the character of Nora sheds the cloak of â€Å"being-for-others† and set out in a search for being-for-self. She says in the end â€Å"we both must be perfectly free†. As Nietzsche’s ideal of human personality combined the virtues of the warrior and the independent thinker and it preferred the difficult life to that of an easy one , in similar way Nora has to leave her shelter and get out in  the open to find her self and think of what she is.Helmer on the other hand can be see as a victim of â€Å"bad faith† who goes on to follow himself identifying too completely and mechanically with a fixed role that absolves him from having to face up to decisions and surrenders to the role which society has coined for him letting the norms dictate him. Ibsen shows the struggle and â€Å"nothingness† of life in his verse play â€Å"Peer Gynt†. The play echo’s many of the characteristics of existentialism. Peer Gynt ,the protagonist of the play , starts out as a highly imaginative, irresponsible youth to find himself only to know in the end that he has no self. He often misquotes proverbs or scripture to justify his actions. The play poses many questions , religious and social , as to how far a man is free to make choices and what kind of choices he has ? The play also asks the pivotal question that what is a man expected to do with is life? There are many instances in the play where Peer has to make a choice . Early on he has to decide whether to become a true man or become a Troll and the end shows the consequences he bears for this choice. Peer moves in a world which is full of uncertainties and he is burdened with the responsibility of shaping his life without anything to hang on to, a lamentation so often cried out by the existentialist playwrights and philosophers. Peer, all through the play is baffled with the choices that he has to make . After he has escapes from the Troll’s Kingdom he says: â€Å"Go in after this? So befouled and disgraced? Go in with that troll-rabble after me still? Speak, yet be silent; confess, yet conceal – â€Å" The stage instructions shows several times his reluctance in moving forward , and small actions mark the important decisions in his life. In the first act when he has to go to the wedding of Ingrid, the girl he had formerly detested , their a series of step taken forward and backward [PEER GYNT comes along a footpath, goes quickly up to the fence, stops, an looks out over the stretch of country below] †¦ [Puts one leg over the fence; then hesitates.] †¦ [Draws back his leg.] By making the choices which he has to make , Peer not only takes the responsibility of his own life but shapes others destinies too. Solveig, the girl who loves him truly, comes to him leaving all that belonged to her and thus she makes her choice , not aware of its consequences. SOLVEIG: The path I have trodden leads back nevermore After he leaves the Troll princes and comes back to Solveig , his true love , and thinks that his life is free from worries , the Troll princes comes to see him. He is again caught in a fix and has to chose between the two , he is now a father and has to bear the consequences of the deeds which he performed earlier in his life. And he has to bear it alone . George Luckas in â€Å"Myth of Nothingness† says: â€Å"The emptiness and hollowness of human life which Sartre calls â€Å"nothingness† is also portrayed in a striking scene in Peer Gynt by Ibsen. The aging Peer Gynt is peeling off the layers of an onion, and playfully compares the single layers with the periods of his life, hoping at the end to come to the core of the onion and the core of his own personality. But layer follows layer, period after period of life; and no core is found.†(1) Jean Paul Sartre’s â€Å"nothingness† also echo’s in the nothingness of Peer Gynt’s life. While talking to the sphinx, he is told that his name means â€Å"The Unknown†. The word â€Å"nothing† occurs twenty-eight times during the course of the play. Peer Gynt is asked several times during the course of the play about the reality of his â€Å"Gyntish-Self † and at one of the points he replies: † the Gyntish Self–it is the host of wishes, appetites, desires,– the Gyntish Self, it is the sea of fancies, exigencies, claims, all that, in short, makes my breast heave, and whereby I, as I, exist.† In fourth act of the play , when he visit Gezih, a village near Cairo, he is asked about his identity and he replies PEER [modestly] † I’ve always endeavoured to be Myself† All his pride comes to an end when he is confronted with the final moment of his life. His meeting with the Button-moulder revleas on him that he has not identity what soever and so he has to be melted down and made into something useful.the fact that he has essentially no identity of his own and is as useless as a button without a loop. â€Å"THE BUTTON-MOULDER Yourself you never have been at all;– then what does it matter, your dying right out? PEER Have I not been–? I could almost laugh! Peer Gynt, then, has been something else, I suppose! No, Button-moulder, you judge in the dark. If you could but look into my very reins, you’d find only Peer there, and Peer all through,– nothing else in the world, no, nor anything more.† Brian Johnston states: Peer finds himself confronting what Jean Paul Sartre stated was the essence of the existential condition: Not only is man what he conceives himself to be, but he is only what he wills himself to be after this first thrust towards existence.Man in nothing else but what he makes of himself. Such is the first principle of existentialism [5] This is the dilemma that Peer confronts in his encounter with the Button Molder, when he is brought up against the realization that he may have no authentic identity at all.(2) Peer has to face the disappointment of his life long struggle when he is confronted with the nothingness of his life and he utters his despair in following words in the play â€Å"So unspeakably poor, then, a soul can go back to nothingness, into the grey of the mist.† Henrik Ibsens plays depict characters like Hedvig, Hedda ,Nora,Peer and Brand show the problems of human beings who do not want to be defined and want to â€Å"stand out†. Citations: 1. Lukà ¡cs, Georg; translated from the German by Henry F. Mins. â€Å"Existentialism†, in: Philosophy for the Future: The Quest of Modern Materialism, edited by Roy Wood Sellars, V.J. McGill, Marvin Farber (New York: The Macmillan Company, 1949) 2. Brain Johnston , â€Å"Love’s Comedy and Peer Gynt† Retrieved : 23th Feburaruy 2004 http://www.ibsenvoyages.com

Black Humor Essay

Is death a laughable matter? Or Christ? Or maybe inhumanity? No. In most situations, people do not laugh at any of those subjects. However, in Kurt Vonnegut’s anti-war novel, Slaughterhouse-Five, he laughs at all of them through the use of black humor. Vonnegut uses black humor as a way to criticize societies in all of his novels, but most notably in Slaughterhouse-Five (Klinkowitz). He uses black humor to criticize peoples’ glorification of war and make humor of death, Christ, and inhumanity. Vonnegut uses an array of situations to ironically make death humorous. Such as when Billy is training to become a solder, his father is shot to death by a friend while deer hunting back home. When Billy is in the hospital recuperating from a plane crash, his wife rushes to the hospital, she has an accident, tears off the exhaust system, arrives at the hospital and dies from carbon monoxide poisoning. Vonnegut uses these examples of situational irony in order to make the reader laugh at such tragedies when really there is nothing to laugh at. After the Dresden fire-bombing Edgar Derby is tried and executed in Dresden, which was firebombed and 135,000 innocent people died in one night, for attempting to steal a teapot. All of these situational ironies and all deaths are narrated by a simple phrase, â€Å"So it goes†. This simple phrase pushes the reader to laugh at the ironic parts of life, even if it is death. And Billy can’t change anything in his life because, â€Å"Among the things Billy Pilgrim could not change were the past, the present and the future† (Vonnegut 60). His happiness or success in his life can only exist in his imagination through his time travels because he knows when and how he will die. The novel ends with the destruction and searching for bodies in Dresden and Billy being released from a prisoner of war status, juxtaposed images of life and death. Because Billy finally is free, he has his life back, whereas all the people in Dresden have their lives taken. The death of thousands and the release of a soldier from POW status are definitely not something humorous, however Vonnegut provides the reader with such contrasting ideas in order to elicit a smile or chuckle. This is complete black humor because black humor is â€Å"combining the morbid and grotesque with humor and farce to give a disturbing effect and convey the absurdity and cruelty of life. † (Black humor) Not only does Vonnegut use black humor to convey death, Vonnegut also criticizes inhumanity through the use of black humor in order to illustrate that death must be accepted but not inhumanity through his use of Roland Weary. Roland Weary is an insane, revenge driven soldier who takes joy in the suffering of others. For example, he feeds a dog a steak with a cut up spring in it in order to get revenge on the dog for biting him. He talks to the dog almost as a child would when he tells the dog â€Å"I’m not mad,† (Vonnegut 139) and states that â€Å"he believed me† (Vonnegut 139). Because Vonnegut portrays Weary as a child by using â€Å"doggy,† which is similar to the diction of a small child, he juxtaposes a childlike character to the inhumanity of the torture that Weary is inflicting on the dog. He compares the innocence of Weary’s diction to Weary’s psychopathic actions. Vonnegut also makes Weary’s inhumanity humorous through his fascination with his triangular blade that â€Å"makes a wound that won’t close up. † (Vonnegut 37) The torture the blade would inflict is made comic by the affection Weary has for it, juxtaposition, between the torture of the blade and the love and obsession Weary has for it, Vonnegut is â€Å"regarding human suffering as absurd rather than pitiable† (Webster’s) in this situation in order to produce such black humor. Vonnegut also laughs at Jesus when most people hold religion to be sacred. Vonnegut describes Christ as â€Å"dead as a doornail† (Vonnegut 203) after he is hung on the cross. This is humorous because Vonnegut compares a huge spiritual icon, Christ, to the bare basics that hold our society together and held Jesus on the cross, nails. The comparison is so ridiculous that it provides a comic purpose and the reader has to laugh at it. The situation also provides a possible source of Billy’s illusion of being unstuck in time, since he is reading about a time traveler in one of Kilgore Trout’s books this may be the point in his life where he gets the idea of being unstuck in time. Vonnegut also goes on to show the lack of interest the Tralfamadorians have in Jesus Christ, the person they are truly interested in is Charles Darwin. Because he taught that â€Å"those who die are meant to die and that corpses are improvements. † (Vonnegut 210) This contrasts the ideas that humans have of death as a negative sad thing and instead teaches that by dying everyone else is better off. This is absurd and humorous because people are devastated by death yet, he is saying that death is great and people benefit from it. Vonnegut uses black humor in many cases in Slaughterhouse-Five to in a way satirize, however he never provides a solution so it is not truly satire. He simply criticizes society and makes the reader think of his or her own solution. Vonnegut wants the reader to realize and act on the idea that the only way we can’t overcome war and deaths in war, is by removing the glorification of killing in war while not providing a solution or way to change the current societal standards.

Dna Analysis Practical Write-Up

Title: DNA analysis Aim: a) Isolate and Purify Bacterial Chromosomal DNA from a strain of E. coli b) Visualization of restriction fragments by Agarose Gel electrophoresis Objectives: * to isolate and purify bacterial chromosomal DNA from a strain of E. coli * to analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different DNA fragments generated by use of molecular markersAbstract This work describes a lysis method for the isolation and purification of bacterial genomic DNA and visualization of the restriction fragments by agarose gel electrophoresis. It was noted that for one to isolate and purify bacterial chromosomal DNA several steps are taken into consideration. DNA was found to absorb at 260nm wavelength in a UV spectrophotometer. Restriction enzymes were added to cleave DNA which would produce various DNA fragments. DNA can be separate d into different sized fragments by gel electrophoresis.The bacterial DNA was successfully isolated and purified however it could not be observed after running the gel. DNA analysis is a standard practice for defining paternity or maternity, predisposition to disease, embryonic health and criminal guilty. But in our context, DNA analysis is mainly used for predisposition of diseases in bacteria. Bacteria are pathogenic microorganisms that cause infectious diseases including cholera, syphilis, anthrax and leprosy. The most common fatal bacterial diseases are respiratory infections such as tuberculosis (Barnum S.R; 1998). Nucleic acids encode information relating to cell structure and function. Cells have the ability to make copies of their DNA and pass this information to daughter cells. Nucleic acids are polymers of nucleotides. Nucleotides are composed of ribose (a 5` carbon) sugar and either a purine and pyrimidine base at 1` position. The purine bases are adenine (A) and guanine (G) and the pyrimidine bases are cytosine (C), thymine (T) and Uracil (U). Uracil is only found in RNA and thymine is only found in DNA (Wiser M. F; 2002).Isolation of nucleic acid – three major types of techniques are employed in the isolation of nucleic acids differential solubility, absorption methods or density gradient centrifugation. The choice of method will depend on the type of DNA being isolated and the application. A major goal of nucleic acid isolation is the removal of proteins. The separation of nucleic acids from proteins is generally accomplished due to their different chemical properties. In particular, the highly charged phosphate backbone makes the nucleic acids rather hydrophilic as compared to proteins which are more hydrophobic (Allison L.A; 2012). Spectrophotometry is a versatile analytical tool. The underlying principle of spectrophotometry is to shine light on a sample and to analyze how the sample affects the light. DNA absorbs light at a wavelength of approximately 260nm (Stryer; 2006). Centrifugation is a process that involves the use of the centrifugal force for the separation of mixtures. Separation is based size, shape and density. It utilizes density difference between the particles/macromolecules and the medium in which these are dispersed (Gupta P. K; 2006).Dispersed systems are subjected to artificially induced gravitational fields. A buffer is an aqueous solution consisting of a mixture of weak acid and its conjugate base or weak base and its conjugate acid. Its pH changes very little when a small amount of strong acid or base is added to it and thus it is used to prevent any change in the pH of a solution (Cowan M. K; 2009). Electrophoresis is a diverse technique of separation used to separate and sometimes purify macromolecules especially proteins and nucleic acids that differ in size, charge or conformation by an electric current (Stryer L. 2006). Gel electrophoresis refers to using a gel as an ant convective mediu m and or sieving medium during electrophoresis. Gel electrophoresis is most commonly used for separation of biological macromolecules such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or protein; however, gel electrophoresis can be used for separation of nanoparticles. Materials Used * Luria Broth medium * SET Buffer * TEN Buffer * Choloroform/isoamyl alcohol. 24:1 mixture * Phenol/ chloroform 1:1 (Buffer saturated phenol) * Ethanol (95%) stored at -20? * Na Acetate * NaCl: 5M sterilized by autoclaving Sodium dodecylsulphate (SDS) : 26% (w/v) * Bacteria cells * Plastic test tubes * Glass rods * Wide bore pipette * Ice bath * Centrifuge * Ethidium bromide * Agarose * TBE buffer Methodology Each group carried out the following procedures: Used two 50ml sterile plastic tubes, harvested cells by centrifugation for 10 min 4’C. Combined pellets to give approximately 1g wet weight of cells. Washed the pellet, re-suspended it in 20ml Ten buffer by gentle vortexing. Harvest ed the cells again as described above. Re suspended the cells in 10ml of Set buffer and let them sit on ice for 5min.Added 1000Â µL of lysozyme and incubated at 37? for 30 min. Divided the cell suspension into two in separate sterile 50ml tubes. Added 5 ml Ten Buffer and 500Â µl of SDS. Gently mixed the tubes by inverting them until lysis occurred. To each tube added 1ml 5M NaCl and an equal volume of buffer saturated phenol. The tubes were inverted till the mixture was emulsified. Separated the phases by centrifugation for 10min at 40C. Recovered the upper aqueous phase using a wide bore pipette. When retaining the aqueous phase the pellicle at the interface was avoided. Repeated the extraction until the interface was clear.Added an equal volume of chloroform and extract residual protein as described above. Transferred the upper aqueous phases from both tubes to a 100ml beaker. Set them on ice and added 1/10th volume 3M Na acetate. Precipitate the DNA by addition of 2 volumes of ice cold 95% ethanol. Mixed thoroughly and allow it to stand for about 5min on ice for the DNA to precipitate. Spooled the DNA out of solution on a glass rod, dipped it into a tube of 95% ethanol and re-suspended in 10ml Ten Buffer. Left to dissolve overnight at 4’C B) Gel electrophoresis The gel was prepared by melting 1. 6g of agarose plus 200ml of 0. x TBE buffer. Swirled the mixture and allowed it to cool to 55?. Added 10? l ethiduim dye Loaded the gel in the following order; 1. Undigested pBSK 2. pBSK + digested with Eco R1 and Xba 1 3. Undigested DNA from a blue colony 4. DNA from a blue colony digested with Eco R1 and Xba 1 5. Undigested DNA from a white colony 6. DNA from a white colony digested with Eco R1 and Xba1 7. Lambda Hind III molecular weight markers After loading the gel it was run at 100 volts for 2 hours. Results We managed to precipitate DNA out of the Bacterial cells. DNA was seen a small white like fragments.However we could not spool the DNA out of sol ution using glass rods due to fact that DNA is a fragile compound hence when we twisted / spooled for DNA we destroyed the DNA strands cutting them into smaller fragments. The following day, analysis of the DNA sample in a spectrophotometer was carried out. It was found that DNA absorbed a specific wavelength of 260nm. This proved the presence of DNA in the sample. Our sample was digested by restriction enzymes and labeled the DNA fragments with an identification dye and ran them on the Gel electrophoresis together with molecular weight markers.After running the gel no observeable bands of different band fragments were observed. Only the molecular weight markers bands were observed. Discussion The TEN and SET buffer were used to lyse the cells. They are good buffering agent, which solubilizes the DNA, while protecting it from degradation. Eluting and storing the DNA in TBE Buffer is helpful if the EDTA does not affect the downstream applications. EDTA chelates or binds to Mg2+ ions present in purified DNA and can help inhibit possible contaminating nuclease activity (Cowan M. K; 2009).Balancing of test tubes before centrifugation in order for the centrifugation process to be effective to create centrifugal field that results in maximum separation of cell components. According to Wiser M. F 2002, DNA is very insoluble in ethanol and isopropanol, but both alcohols are very water soluble. Thus, it will dissolve in water to form a solution and cause the DNA in the solution to aggregate and precipitate out. Isopropanol is often better to use because it has greater potency in precipitating the DNA and thus lower concentration is required. This is advantageous because it will take less time for the isopropyl alcohol to evaporate.Salts such as sodium chloride and ammonium acetate remove histone and non-histone chromosomal proteins bound to the DNA. As soon as 95% ethanol was added after sodium acetate for DNA precipitation, the whole solution turned cloudy with a lot of white precipitate, precipitating down. According to Allison L. A, 2012; sodium acetate which is negatively charged and low pH was used which contributes to charging positively the DNA. A combination of this plus high salt molarity enhances formation of aggregates of DNA and facilitates the pelleting procedure. Chloroform isoamyl-alcohol is a type of detergent.It binds to protein and lipids of cell membrane and dissolves them. By this it disrupted the bonds that hold the cell membrane together and cause it to breakdown. It then forms complexes with these lipids and proteins, causing them to precipitate out of solution (Besty T and Keogh J; 2005). This reduced chance of contaminated DNA being obtained hence making it possible for us to be able to precipitate DNA only. Alcohol (95%ethanol) is used to precipitate DNA. SDS which stands for ‘sodium dodecyl sulfate' is a strong anionic detergent that can solubilize the proteins and lipids that form the membranes.This will helped t he cell membranes and nuclear envelopes to break down and expose the chromosomes that contain the DNA. In addition to removing the membrane barriers, SDS helped release the DNA from histones and other DNA binding proteins by denaturing them (Barnum S. R; 1998). Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. When exposed to ultraviolet light, it will fluoresce with an orange colour, intensifying almost 20-fold after binding to DNA (Wiser M.F; 2012). Molecular weight size is a set of standards that are used to identify the approximate size of a molecule run on a gel. These markers were composed of nucleic acids of different sizes. A few reasons you may not see bands on the gel after electrophoresis: When preparing the gel for electrophoresis TBE buffer was used. This was done so that the temperature can be maintained and lubricate the electrolyte. L oading dye was added this helped weigh down the DNA so that it can sink into the bottom wells and not float in the buffer solution. According to Gupta P.K, 2006; loading dye moves quickly than the actual DNA parts so it is an indicator to when to turn off the power on the electrophoresis chamber. The dye also makes the DNA visible to the naked eye, giving it a purplish color and making it easier to work with. After Gel electrophoresis no bands of DNA were observed. This according Allison L. A (2012) might have been as a result of any of the following * DNA concentration might have been too low. * DNA sample is contaminated with RNA and Protein * DNA bands are too small and have run out of the gel The buffer system in which the gel is suspended is not doing its job correctly. The buffer might have to be made fresh. * The electrophoresis apparatus is not in the correct orientation (electrodes not connected to the right poles). The major drawback in the experiment was that our fellow c olleagues were not able to isolate and purify their DNA. Also when working with DNA temperature regulations were not sometimes adhered to, it was sometimes left on the surface tables for long periods esp. when the samples were being analyzed in the spectrophotometer.Recommendations With proper teamwork and co-ordination among my fellow classmates much larger quantities of DNA could have been isolated and purified. The DNA should not be kept at room conditions for a long time. Conclusion The experiment was partly a success managed to isolate and purify DNA, analyzed it using a spectrophotometer. However bands of DNA could not be visualized after running the gel. References 1. Allison L. A. (2012). Fundamental Molecular Biology, 2nd edition. Denvers. John Wiley and Sons Inc. 2. Barnum Susan.R, (1998), Biotechnology: An introduction, New Delhi, Vikas Publishing House. 3. Besty Tom and Keogh Jim, (2005), Microbiology demystified, New York; MacGraw-Hill. 4. Cowan Majorie Kelly, (2009), M icrobiology: A Systems Approach, 3rd edition; New York; MacGraw-Hill. 5. Gupta, P. K. (2006). Elements of Biotechnology, Meerut. Rastogi Publications. 6. Stryer L, Berg J. M and John Tymozcko. (2006). Biochemistry. 5th edition. California. W. H Freeman and Company. 7. Wiser, M. F. (2002). Methods in cell biology. Berlin. Springer Verlog CHINHOYI UNIVERSITY OF TECHNOLOGYName Tanyaradzwa R Ngara Reg Number C1110934J Course Recombinant DNA Technology Module Code CUBT 203 Program Biotechnology Level 2:1 Lecturer Dr Mlambo Practical Write-up DNA analysis

Parenting Skills Essay

What financial needs are parents obligated to provide and which are optional? The financial needs that parents are obligated to provide include nutritious food, shelter, clothing, and health care. The ones that are optional are luxury items such as summer classes, piano lessons, or various electronic items. 3. What other needs might a child have that a parent is expected to provide? Parents are also required to provide safety and security for their child. 4. What are the qualities of a nurturing parent? Qualities of a nurturing parent include retaining a healthy self-esteem so that their child has a basis for self-esteem, a sense of empathy for their child, a way to successfully enforce disciplinary codes, and an understanding of acceptable maturity levels for their child. Critical Thinking Questions 1. What qualities make a person a good parent? A bad parent? The qualities that make a good parent are listening skills, understanding of maturity levels, reasonable mind, and a good education and career. Qualities that make a bad parent include a bad temper or short temper, low education, and ideas of achieving their own goals above their child’s. 2. Which parental responsibilities do you think would be the most challenging? Why? The parental responsibilities that I think would be the most challenging would be having a job that is good enough to afford a child. This is because it is hard to maintain a good job while having a child and still be there for the child emotionally and physically while bringing in a decent paycheck. 3. Which parental responsibilities do you think would be the most interesting to you? Why? The parental responsibilities that I think will be the most interesting to me the education of life skills. It is interesting to think that a child picks up on habits so easily and they can use those skills for the rest of their life. 4. What are some things that people can do to prepare for parenthood? Some things people can do to prepare for parenthood is further their education and get a career. Also, they need to make sure both parties in the relationship want a child. However, no one is ever truly prepared for a child.

Organizational Analysis Essay Example | Topics and Well Written Essays - 2000 words

Organizational Analysis - Essay Example Being aware of the major differences in a group and maintaining patience can help minimize such problems. Having worked in a team during this season, I experienced some challenges, problems, and conflicts that required sound decision making and problem solving skills as per the context in which the occurred. Our research team was given the task to deliver case studies and exhibitions of the module in an excellent way. The challenge was realized in building a good relation and communication network between my team members. This was due the differences in our cultural backgrounds. I am from Qatar, yet all my group members are of Chinese origin. Many people assume that challenges in cross-cultural teams arise from dissimilar styles of communication, yet this is just one of the many challenges that can create obstacles to the ultimate success of a team. These categories of challenges include direct versus indirect communication, differing attitudes towards authority and leadership, trouble with fluency and accents, and conflicting norms in making decisions (Minssen, 2006: 14). In the context of my group, I realized that the challenge in communication was mainly based on the principle of direct versus in direct communication (Trenholm & Jensen, 2008: 13). Qatar being an Arabian country, I always thought that communication in Arabian culture is typically explicit and direct. The meaning of words is on the surface and the receiver of the message does not have to have much about the context of communication or the speaker to make an interpretation. This is not true in the Chinese culture, where I found that the meaning of words is embedded in the manner in which the message is presented. The challenge was mostly due to the indirect communication that I noticed among them. For instance, my Chinese team members would often take a break and have some conversations in the middle of discussions. Little did I know that their main aim was to maintain harmony in

Simultaneous determination of eight illegal dyes in chili products by Coursework

Simultaneous determination of eight illegal dyes in chili products by liquid chromatography-tandem mass spectrometry - Coursework Example Cross- contamination or adulteration is one of the main foods contaminating process in the due use of Sudan dyes (Juan Lia et al. 2013). This experiment ought to have taken pre-cautions to inform its readers on the harmful effects of these eight illegal dyes in chili products. Spectrophotometric method could have been discussed somewhere in this paper as a way that can be used to detect the presence of Sudan dyes and other illegal dyes in chili powders or any other food stuff in ppm levels (Juan Lia et al. 2013). This method has been found reliable in identifying and estimating the level of sudan I-IV in Chili samples. Ethyl acetate is yet another useful solvent used to extract dyes from chili samples as well as the preparation of samples. The eight illegal dyes discussed in this paper cause carcinogenicity which is a high producing capacity among many other ailments. Otherwise, the paper developed the HPLC-MS/MS chronologically from the first to the last step demonstrating the extraction procedure used to determine eight illegal synthetic dyes in chili products simultaneously. The selection of MS/MS technique combined with chromatographic separation was an accurate method of determining compounds in complex matrices with less ambiguity (Juan Lia et al. 2013). The method was effective that it did not require the laborious clean up procedures. The method was highly accurate with a good repeatability. The detection of any limits like quantification limits was minimal (Juan Lia et al. 2013). The HPLC-MS/MS process employed in the paper proved to be effective for fraud detections in both exported and imported chili products. The analysis of data was done effectively through the use of graphs and tables to illustrate the results. The chemical structures of the eight illegal dyes analysed were also illustrated in both

The role of the spectator Thesis Proposal Example | Topics and Well Written Essays - 1250 words - 1

The role of the spectator - Thesis Proposal Example Apparently, the passive spectator is a quite new state of art experience, but however has become quite widespread that it is the status quo for the majority of art performances in the West. The passive spectator actually only emerge in the nineteenth century, as art performances initiated its separation into entertainment and artistic forms. Professionals and scholars such as Wagner, with he and Henry Irving with their murky theater, and his ‘mystic chasm’, began several of the numerous initiatives in the nineteenth century that concretely detached the spectator from the performance and dampened ‘spectatorial acts of ownership or displeasure’, or even loud agreement. This projection of the passive spectator has become quite recognized that in a 1991 article in New York Times on the ill behaviors of theatre spectators, the journalist Alex Witchel did not include the rationale underlying his request for courteousness or admit that genuinely good theatre usually moves or stirs in its audiences just the contrary of the passive silence he wanted. This courteous, overwhelmed response has become the standard. However, we are at a decisive period in performance studies in which we have to perceive contemporary performance beyond the very same borders from which it is breaking out. This study argues that it is wholly impossible to observe and study contemporary performance while remaining grounded on the perspective as the traditional audience. Not merely do we have to keep on creating and experimenting new performance tactics, but we also have to move our focus to ‘response strategies’, to directly reconstruct the role of the spectator as a contemporary audience. I request the contemporary audience to become engaged in a new form of agreement with the creators of new performance. Through this study, I request this contemporary audience for several things: to take

Improving Driver Safety Essay Example | Topics and Well Written Essays - 500 words

Improving Driver Safety - Essay Example Teens who start driving are excited about the freedom that driving gives them, and are less inclined to think seriously about safety issues. Communicating safety messages to teens can be problematic, simply because they're teenagers and may chafe at what they see as restrictions on their freedom. This means that to improve teen driving safety, education programs must deliver safety messages in ways that are specifically targeted to teens and which do not make them feel they are being 'talked down to'. Driving education programs for teenagers should be designed to stimulate interest in road safety and should clearly show them the possible consequences of unsafe driving. To help keep teens interested in the program, a range of activities should be designed that are fun and interactive while sending clear messages about the importance of road safety. Activities can include practical demonstrations which teens can take part in, and interactive film clips in which they can make choices for the characters portrayed. Educating teens on the practice of safe driving will include information on speeding, seat belts, drinking and driving, and how to handle distractions while driving.

Are female suicide bombers rational actors Research Paper

Are female suicide bombers rational actors - Research Paper Example The major advantage of suicide bombing attack is the accuracy and precision in conducting secret missions. Under normal circumstances, nobody would like to sacrifice their lives for any kind of missions; however the influence of fundamentalism and patriotism is getting increased day by day and because of that many people come forward to sacrifice their lives for safeguarding their traditions, culture and beliefs. Even though all types of suicide bomber attacks are difficult to resist, female suicide bombing attacks seem to be the most difficult type of attacks as far as preventive measures are concerned. Genetically and historically, it was believed that males were more aggressive in nature whereas females were more of peace loving nature. However, the above belief or theory seems to be less popular now because of the readiness of current females in taking part in risky missions and activities. As per some of the recent reports available from states like Iraq, Palestine, Sri Lanka etc, the number of female suicide bombers is increasing day by day. â€Å"While arguing about why women fight, many believe that these women are yet other victims in the hands of ruthless men while others emphasize the seriousness of a particular conflict in which even women are driven towards taking up arms, seen as a last resort in the eyes of many†. In short, there are different viewpoints about the reasons why females are increasingly participating in suicide bombing attacks.... Some people argue that suicide bombing attacks in general and female suicide bombing attacks in particular are motivated by irrational motives. However, many others believe that strong rational motives are behind every female suicide bombing attacks. This paper argues that female suicide bombers are rational actors and they engage in terrorist activities not because of the influence of any external agents alone, but because of their own instincts and judgements. Religious extremism is cited as the major reason for increasing female suicide attacks by many of the sociologists and politicians. It is because of the fact that most of the female suicide bombers are from Muslim community. In countries like Palestine, Iraq, Afghanistan, etc Muslim female suicide bombers are causing big problems and headaches to Israelis and Americans. Jihad or sacred war against nonbelievers is accepted as a tradition by many of the Muslim militant groups. At the same time it should be noted that jihad is m ostly taking place in countries under some kind of foreign occupation. The occupation of Israelis in Palestine and Americans in Iraq and Afghanistan can be cited as the best examples for the above belief. In other words, foreign occupation seems to be one of the major reasons for increased number of suicide attacks. Since women participation in modern social and political activities are increasing day by day, it is difficult for them to stay away from helping male community in their efforts to fight against all kind of foreign occupations. Female suicide bombers are thus utilized for pressurizing foreign occupiers to withdraw their troops from their land by the militant groups- in that respect; it is not an irrational activity,

Powering service workers at Harvester Restaurants. Personnel Review Assignment

Powering service workers at Harvester Restaurants. Personnel Review - Assignment Example TASK C – Strategic Fit Analysis†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦12 4a) Segmentation  Strategy†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦12 4b) Marketing strategy†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦.13 4c) Advertising strategy†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦13 4d) Branding†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦...14 4e) Selling†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚ ¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦14 4f) Manufacturing†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦..14 4g) Human resource strategy†¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦15 References NIKE 1. Introduction: Nike Inc was founded in 1962 by two partners Bill Bowerman and Phil Knight. The name was originally Blue Ribbon Sports. The goal then was to start a business that broke the domination of German sportswear in the domestic American retail. Market regions have expanded to more than 100 countries in regions like United States, Europe, Asia pacific and the Caribbean. Distribution channels have expanded into both traditional and non tradit ional ones with social media marketing also increasing. It is the world’s leading supplier of athletic shoes and apparel and also a manufacturer of sports equipments. For 2009 the revenue was about $19.2 billion and employees amounted to 30,000. The company’s name was changed to Nike Inc in 1978; it is derived from the Greek goddess of victory’s name. the other brands Nike has are: Nike Golf, Nike Pro,  Nike+,  Air Jordan,  Nike Skateboarding, and subsidiaries including Cole,  Hurley International,  Umbro  and  Converse. Nike’s sport ambassadors are huge personalities, from Michael Jordan to Roger Federer that further promote their products. The ‘swoosh’ logo and the slogan ‘Just do it’ has become recognizable everywhere due to the company’s worldwide presence and quality goods. Products: Primary product is athletic footwear and athletic apparel. Newer product offerings include Performance equipments like bal ls, timepieces, eyewear, bats and other equipments designed for sporting activities. Product categories are: Running Basketball Cross-Training Outdoor Activities Tennis Golf Soccer Baseball Football Bicycling Volleyball Wrestling Cheerleading Aquatic Activities Auto Racing Other athletic and recreational uses Phil Knight was responsible for entering into their fist ever agreement with a Japanese manufacturing company named Onitsuka Tiger company. They were to be the sole supplier of BRS in the United States of high quality athletic shoes. The first worldwide successful shoe design was Cortez designed by both Bowerman and Tiger and it was sold at the first BRS store. In 1972 however Tiger left the company and the company changed its name to Nike inc. in the late 70s, famous sportspeople started wearing the shoes and the company was made famous. In 1980 Nike went public on the New York stock exchange. 2. TASK A – Market Environment Analysis   ANALYSIS: 2a) Environmental Analy sis Internal – Strength Nike’s own management style, its history, its product range all are strengths that the company utilizes. They have

Prevention and Control of Diseases and Health Conditions Assignment

Prevention and Control of Diseases and Health Conditions - Assignment Example In the time of menopause, women experiences declining level of progesterone and estrogen thus having symptomatic changes for women. However, HRT can be an efficient treatment for the typical menopause symptoms. According to studies on HRT, they should that the risk: benefit ratio can rise significantly for those women with ages 60 years and above. The risk involved such as stroke, breast cancer, endometrial cancer, ovarian cancer among others complications might have been the main thing that went wrong in the clinical studies (OConnor-Fleming, & Parker, 2008). HPV is said to be the most common American’s sexually transmitted disease. Merck Pharmaceuticals having introduced a vaccine that protects against the sexually transmitted virus raises many concerns, not on the issue of risks but on the issue of ethics. In my opinion, I think the vaccine will make things worse rather than good. On the issue of ethics, the vaccine will lead to some women letting their guard down when it concerns annual gynecological exams and safe sex (McKenzie, Pinger, & Kotecki, 2012). On the issue of breast cancer, the age bracket is not relevant considering the past statistics on the range of women who have died of breast cancer. According to the Canadian National Breast Screening Study, 5% of the screenings are positive or suspicious, but the 80-93 % is false positives that cause anxiety to many, however, on public perspective, this shows that the hospitals undertaking the screening might be using tests have questionable accuracy. I believe the 5% is worth saving, considering the limited resources and screening in any age group is not justifiable (OConnor-Fleming, & Parker,

The cornerstones of character Essay Example for Free

The cornerstones of character Essay Challenges are the cornerstones of character. They are natural parts of life that not only support change, but they also strengthen the mold of which we are made. Of the many forms challenges that appear, two challenges (one of which is inevitable and the other that is self-imposed) do their parts to alter who we are for the better. Though a natural part of life, death, in any form, evokes and arouses many conflicting emotions. Experiencing my grandmother’s death at the age of sixteen marked the first time I attended a funeral. The fact that it was the funeral of someone very dear to me made my first attendance extremely difficult. The black dresses and solemn looks mirrored the emotions I felt inside. The beautiful remarks about my grandmother did little to comfort me or provide supplement for the inner loss I felt. Moreover, knowing I had to let go continuously battled the potent feeling of not wanting to let go. While old enough to realize that death is a natural end to life that must come at some point, I was also cognizant of the fact that death had marked an end to a very valuable friendship. My grandmother was the epitome of wisdom. Though her words of wisdom will forever resonate in my mind, I will deeply continue to miss her reassuring voice and still harbor a bit of resentment towards death for permanently depriving me of time in the future that I could have spent listening and learning from more of her valuable advice. One lesson learned from my grandmother’s death is to seize and savor each present moment. A newspaper article said that the present is so appropriately named because it is a â€Å"gift†. Gifts are meant to provide pleasure on more than one occasion. Thus, I have learned to assume and appreciate the times with my grandmother as invaluable and lasting sentiments. Approximately one year after my grandmother’s death, my idol and role model announced that life was too hard for him to continue. Working full time and attending medical school proved to be too challenging for my brother. He would go to classes during the day, take short naps, and whisk off to his night job. Though his goals were noble and definitely attainable, my brother failed to recognize his limits. Going to class required usage from the mental power source, for which a couple of hours of sleep could not replenish. The job required more mental effort as well as physical effort. As a result of his self-imposed ideals, my brother reached a point of exhaustion that threatened his logic and life. Between the two demands, my brother cracked under the pressure. He had simply stretched himself too thin. Witnessing my brother’s breakdown taught me that having high goals is acceptable, but that reasonable planning to reach those goals is just as important. When pursued logically and strategically, high goals will still challenge, but they will also prompt growth. On the other hand, pursuing elevated goals irrationally results in unnecessary stress, which results in a despondent state of mind and eventually death. Though one goal takes precedence over the other, my brother did not have to completely abandon one for the sake of the other. School was definitely a priority that could not be compromised. On the other hand, his job could have been part-time instead of full-time. Obviously, my brother was not going to make a sufficient amount of money working his current job; therefore, the number of hours were not as important as school. By continuing to pursue school as a full-time endeavor and reducing his work hours, my brother found a functional medium that allowed him to better balance both endeavors. Life is a gift of challenges that condition and a reward that serve as reminders. The challenges are there to strengthen us and aid in our growth to become better individuals. In addition, those challenges help us to recognize and appreciate the rewards. Though each serves in a different capacity, both should be taken with great care. Without either, we would be stuck in a stagnant pool of convenience surrounded by false senses of hope and security.